Journal: Cellular Oncology
Article Title: Targeting senescence-like tumor-associated macrophages sensitizes chemotherapy in triple-negative breast cancer
doi: 10.1007/s13402-026-01197-3
Figure Lengend Snippet: Senescence-like TAMs in clinical specimens. (A) IF analysis of p16 + CD163 + TAMs in treatment-naïve TNBC (direct surgery, n = 20) versus post-neoadjuvant chemotherapy specimens ( n = 17). (B) IF analysis of p16 + CD163 + TAMs in post-chemotherapy samples from patients with progressive disease (PD, n = 5) versus complete/partial remission (CR/PR, n = 12). (C) The senescence-like TAM gene signature scores across different sample groups: complete response, partial response, stable disease, and progressive disease. Data was from the BRCA-TCGA cohort and analyzed by GEPIA3 ( https://gepia3.bioinfoliu.com/ ). (D) The prognostic value of senescence-like TAM gene signature in cases with Carboplatin treatment. For statistical analyses, an unpaired two-tailed t-test (A and B) or log-rank test (E) was used. ** P < 0.01 and **** P < 0.0001
Article Snippet: Endothelial cells were enriched by magnetic-activated cell sorting using the CD31 MicroBead Kit (Miltenyi Biotec Cat# 130-091-935, RRID: AB_3699142), TAMs were enriched with the CD163 MicroBead Kit (Miltenyi Biotec, Cat# 130-124-420), and CAFs were enriched using Anti-Fibroblast MicroBeads (Miltenyi Biotec, Cat# 130-050-601), in accordance with the manufacturers’ protocols.
Techniques: Two Tailed Test